The ability of sera to sensitize basophils or the mast cell line to Ara h 2 was also dependent on recognition of the whole protein in the Western Blots (Figure 1.1). This peptide, a known IgE binding sequence from Ara h 2, could somewhat inhibit degranulation by Ara h 2 from the LAD2 mast cell line. There were also difficulties with solubility of the peptides but work was done with the most soluble, peptide 1, as of this progress report. With the high prevalence of peanut and tree nut allergy in the US and concern for potential cross-reactivity with sesame, allergists suggest screening peanut and tree nut allergic children for sesame allergy ( 8 ). Originally, the grant had proposed use of human basophils, however, the variability between donors was too high to visualize the slight differences in basophil activation that could be expected by inhibition of only one IgE epitope using the synthesized peptides. The peptides described in Aim 2 are undergoing evaluation of their ability to inhibit degranulation of a mast cell line and basophil cell line. Individuals may experience allergic reactions to foods, insect venom, latex, and a variety of environmental exposures. It was clear that the walnut sensitive individuals recognized sequences selected from walnut allergens better than the patients who were sensitive only to peanuts, or cross-reactive with a mild allergy to walnut. Aim 3 : The data were observed in terms of the degree of cross reactivity of the patients for peanuts and tree nuts. The physician is called on to determine the risk of reaction to related foods among legumes, tree nuts, fish, shellfish, cereal grains, mammalian and avian food products, and a variety of other plant-derived foods that may share proteins with pollens, latex, and each other.
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